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In this B subtilis strain (AJ 1992 from Ajinomoto, Japan) glucose is utilized to form pyruvate, which in the presence of excess oxygen is completely oxidized to carbon dioxide and water via the trichloroacetic and (TCA) and respiratory cycles At low oxygen levels, pyruvate is used to produce acetoin, which in turn can be reduced by nicotinamide adenine dinucleotide (NADH) to form butanediol Lactate is also formed from pyruvate at low levels of oxygen Acetoin is produced primarily at oxygen levels above 150 parts per billion (ppb) and butanediol below 80 ppb Moes et al (1985) demonstrated the extreme sensitivity of the culture in the range 80 to 90 ppb Using a complex medium, batch fermentations could be completed within 8 h with a biomass concentration between 2 to 3 g/L, using approximately 11 g/L of glucose. You can change any of the information associated with your local site roots by choosing Site Manage Sites. 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Note For oxygen concentrations above 100 ppb, typical values of acetoin and butanediol concentrations were in the range 0 to 3 and 0 to 05 g/L, respectively At low oxygen concentrations (50 ppb), values of acetoin and butanediol were in the range 0 to 5 and 0 to 3 g/L, respectively In batch fermentations, switching from one oxygen level to another caused one already accumulated product to be converted to the other in a reversible manner The high rates of change of 05 to 10 g/L per hour enabled detection within 10 min Griot et al (1986, 1987) showed that this strain changed the selectivity of excretion of metabolites in response to variations in dissolved oxygen levels in less than 1 s The response time of the culture to a change in gas phase oxygen concentration was shown using mass spectrometry to be a total of 7. Click Rename. Fireworks displays the Save Command dialog box. Enter a new name for the command, and click OK to rename it. 3. 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